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transcriptor reverse transcriptase enzyme  (Qiagen)


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    Structured Review

    Qiagen transcriptor reverse transcriptase enzyme
    Transcriptor Reverse Transcriptase Enzyme, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 6786 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptor+reverse+transcriptase+enzyme/Reverse+transcriptase/pmc02662061-112-100-92
    Average 97 stars, based on 6786 article reviews
    transcriptor reverse transcriptase enzyme - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    TA Cloning:

    Article Title: RNA Editing of Androgen Receptor Gene Transcripts in Prostate Cancer Cells
    Article Snippet: .. The following reagents were purchased: AR agonist R1881 (methyltrienolone) (PerkinElmer Life Sciences); 17β-estradiol, progesterone, cyproterone acetate (CPA), hydroxyflutamide (HF) (Sigma); double-stranded siRNAs (Dharmacon Research, Lafayette, CO); Oligofectamine reagent, 4–12% SDS-polyacrylamide gels, and the TOPO TA cloning kit (Invitrogen); prestained Precision Plus protein standards and goat anti-mouse horseradish peroxidase-conjugated secondary (Bio-Rad); mouse monoclonal AR antibody (AR441) (Santa Cruz Biotechnology (Santa Cruz, CA); MEGAscript® high yield transcription kit (Ambion, Austin, TX); BCA protein assay kit (Pierce); ECL reagents kit and Hyperfilm ECL film (GE Healthcare); RNeasy midi kit, Oligotex Midi kit, and DNA oligonucleotides (Qiagen, Valencia, CA); proteinase K solution, DNase-free RNase, transcriptor reverse transcriptase enzyme, and FastStart Taq DNA polymerase (Roche Applied Science); fetal bovine serum and charcoal-stripped fetal bovine serum (Hyclone Laboratories, Logan, UT); fluorescent short tandem repeat (STR) oligonucleotides (Applied Biosystems, Foster City, CA); GeneRuler 1-kb DNA ladder (MBI Fermentas, Hanover, MD); rat genomic DNA (ATCC, Manassas, VA); and qPCR primers (SuperArray, Frederick, MD). ..

    Article Title: RNA Editing of Androgen Receptor Gene Transcripts in Prostate Cancer Cells
    Article Snippet: .. The following reagents were purchased: AR agonist R1881 (methyltrienolone) (PerkinElmer Life Sciences); 17 -estradiol, progesterone, cyproterone acetate (CPA), hydroxyflutamide (HF) (Sigma); double-stranded siRNAs (Dharmacon Research, Lafayette, CO);Oligofectamine reagent, 4–12%SDS-polyacrylamide gels, and the TOPO TA cloning kit (Invitrogen); prestained Precision Plus protein standards and goat antimouse horseradish peroxidase-conjugated secondary (BioRad); mouse monoclonal AR antibody (AR441) (Santa Cruz Biotechnology (Santa Cruz, CA);MEGAscript high yield transcription kit (Ambion, Austin, TX); BCA protein assay kit (Pierce); ECL reagents kit and Hyperfilm ECL film (GE Healthcare); RNeasy midi kit, Oligotex Midi kit, and DNA oligonucleotides (Qiagen, Valencia, CA); proteinase K solution, DNase-free RNase, transcriptor reverse transcriptase enzyme, and FastStart TaqDNA polymerase (Roche Applied Science); fetal bovine serum and charcoal-stripped fetal bovine serum (Hyclone Laboratories, Logan, UT); fluorescent short tandem repeat (STR) oligonucleotides (Applied Biosystems, Foster City, CA); GeneRuler 1-kb DNA ladder (MBI Fermentas, Hanover, MD); rat genomic DNA (ATCC, Manassas, VA); and qPCR primers (SuperArray, Frederick, MD). ..

    Article Title: RNA Editing of Androgen Receptor Gene Transcripts in Prostate Cancer Cells
    Article Snippet: .. Reagents The following reagents were purchased: AR agonist R1881 (methyltrienolone) (PerkinElmer Life Sciences); 17β-estradiol, progesterone, cyproterone acetate (CPA), hydroxyflutamide (HF) (Sigma); double-stranded siRNAs (Dharmacon Research, Lafayette, CO); Oligofectamine reagent, 4–12% SDS-polyacrylamide gels, and the TOPO TA cloning kit (Invitrogen); prestained Precision Plus protein standards and goat anti-mouse horseradish peroxidase-conjugated secondary (Bio-Rad); mouse monoclonal AR antibody (AR441) (Santa Cruz Biotechnology (Santa Cruz, CA); MEGAscript® high yield transcription kit (Ambion, Austin, TX); BCA protein assay kit (Pierce); ECL reagents kit and Hyperfilm ECL film (GE Healthcare); RNeasy midi kit, Oligotex Midi kit, and DNA oligonucleotides (Qiagen, Valencia, CA); proteinase K solution, DNase-free RNase, transcriptor reverse transcriptase enzyme, and FastStart Taq DNA polymerase (Roche Applied Science); fetal bovine serum and charcoal-stripped fetal bovine serum (Hyclone Laboratories, Logan, UT); fluorescent short tandem repeat (STR) oligonucleotides (Applied Biosystems, Foster City, CA); GeneRuler 1-kb DNA ladder (MBI Fermentas, Hanover, MD); rat genomic DNA (ATCC, Manassas, VA); and qPCR primers (SuperArray, Frederick, MD). ..

    Bicinchoninic Acid Protein Assay:

    Article Title: RNA Editing of Androgen Receptor Gene Transcripts in Prostate Cancer Cells
    Article Snippet: .. The following reagents were purchased: AR agonist R1881 (methyltrienolone) (PerkinElmer Life Sciences); 17β-estradiol, progesterone, cyproterone acetate (CPA), hydroxyflutamide (HF) (Sigma); double-stranded siRNAs (Dharmacon Research, Lafayette, CO); Oligofectamine reagent, 4–12% SDS-polyacrylamide gels, and the TOPO TA cloning kit (Invitrogen); prestained Precision Plus protein standards and goat anti-mouse horseradish peroxidase-conjugated secondary (Bio-Rad); mouse monoclonal AR antibody (AR441) (Santa Cruz Biotechnology (Santa Cruz, CA); MEGAscript® high yield transcription kit (Ambion, Austin, TX); BCA protein assay kit (Pierce); ECL reagents kit and Hyperfilm ECL film (GE Healthcare); RNeasy midi kit, Oligotex Midi kit, and DNA oligonucleotides (Qiagen, Valencia, CA); proteinase K solution, DNase-free RNase, transcriptor reverse transcriptase enzyme, and FastStart Taq DNA polymerase (Roche Applied Science); fetal bovine serum and charcoal-stripped fetal bovine serum (Hyclone Laboratories, Logan, UT); fluorescent short tandem repeat (STR) oligonucleotides (Applied Biosystems, Foster City, CA); GeneRuler 1-kb DNA ladder (MBI Fermentas, Hanover, MD); rat genomic DNA (ATCC, Manassas, VA); and qPCR primers (SuperArray, Frederick, MD). ..

    Article Title: RNA Editing of Androgen Receptor Gene Transcripts in Prostate Cancer Cells
    Article Snippet: .. The following reagents were purchased: AR agonist R1881 (methyltrienolone) (PerkinElmer Life Sciences); 17 -estradiol, progesterone, cyproterone acetate (CPA), hydroxyflutamide (HF) (Sigma); double-stranded siRNAs (Dharmacon Research, Lafayette, CO);Oligofectamine reagent, 4–12%SDS-polyacrylamide gels, and the TOPO TA cloning kit (Invitrogen); prestained Precision Plus protein standards and goat antimouse horseradish peroxidase-conjugated secondary (BioRad); mouse monoclonal AR antibody (AR441) (Santa Cruz Biotechnology (Santa Cruz, CA);MEGAscript high yield transcription kit (Ambion, Austin, TX); BCA protein assay kit (Pierce); ECL reagents kit and Hyperfilm ECL film (GE Healthcare); RNeasy midi kit, Oligotex Midi kit, and DNA oligonucleotides (Qiagen, Valencia, CA); proteinase K solution, DNase-free RNase, transcriptor reverse transcriptase enzyme, and FastStart TaqDNA polymerase (Roche Applied Science); fetal bovine serum and charcoal-stripped fetal bovine serum (Hyclone Laboratories, Logan, UT); fluorescent short tandem repeat (STR) oligonucleotides (Applied Biosystems, Foster City, CA); GeneRuler 1-kb DNA ladder (MBI Fermentas, Hanover, MD); rat genomic DNA (ATCC, Manassas, VA); and qPCR primers (SuperArray, Frederick, MD). ..

    Article Title: RNA Editing of Androgen Receptor Gene Transcripts in Prostate Cancer Cells
    Article Snippet: .. Reagents The following reagents were purchased: AR agonist R1881 (methyltrienolone) (PerkinElmer Life Sciences); 17β-estradiol, progesterone, cyproterone acetate (CPA), hydroxyflutamide (HF) (Sigma); double-stranded siRNAs (Dharmacon Research, Lafayette, CO); Oligofectamine reagent, 4–12% SDS-polyacrylamide gels, and the TOPO TA cloning kit (Invitrogen); prestained Precision Plus protein standards and goat anti-mouse horseradish peroxidase-conjugated secondary (Bio-Rad); mouse monoclonal AR antibody (AR441) (Santa Cruz Biotechnology (Santa Cruz, CA); MEGAscript® high yield transcription kit (Ambion, Austin, TX); BCA protein assay kit (Pierce); ECL reagents kit and Hyperfilm ECL film (GE Healthcare); RNeasy midi kit, Oligotex Midi kit, and DNA oligonucleotides (Qiagen, Valencia, CA); proteinase K solution, DNase-free RNase, transcriptor reverse transcriptase enzyme, and FastStart Taq DNA polymerase (Roche Applied Science); fetal bovine serum and charcoal-stripped fetal bovine serum (Hyclone Laboratories, Logan, UT); fluorescent short tandem repeat (STR) oligonucleotides (Applied Biosystems, Foster City, CA); GeneRuler 1-kb DNA ladder (MBI Fermentas, Hanover, MD); rat genomic DNA (ATCC, Manassas, VA); and qPCR primers (SuperArray, Frederick, MD). ..

    Reverse Transcription:

    Article Title: RNA Editing of Androgen Receptor Gene Transcripts in Prostate Cancer Cells
    Article Snippet: .. The following reagents were purchased: AR agonist R1881 (methyltrienolone) (PerkinElmer Life Sciences); 17β-estradiol, progesterone, cyproterone acetate (CPA), hydroxyflutamide (HF) (Sigma); double-stranded siRNAs (Dharmacon Research, Lafayette, CO); Oligofectamine reagent, 4–12% SDS-polyacrylamide gels, and the TOPO TA cloning kit (Invitrogen); prestained Precision Plus protein standards and goat anti-mouse horseradish peroxidase-conjugated secondary (Bio-Rad); mouse monoclonal AR antibody (AR441) (Santa Cruz Biotechnology (Santa Cruz, CA); MEGAscript® high yield transcription kit (Ambion, Austin, TX); BCA protein assay kit (Pierce); ECL reagents kit and Hyperfilm ECL film (GE Healthcare); RNeasy midi kit, Oligotex Midi kit, and DNA oligonucleotides (Qiagen, Valencia, CA); proteinase K solution, DNase-free RNase, transcriptor reverse transcriptase enzyme, and FastStart Taq DNA polymerase (Roche Applied Science); fetal bovine serum and charcoal-stripped fetal bovine serum (Hyclone Laboratories, Logan, UT); fluorescent short tandem repeat (STR) oligonucleotides (Applied Biosystems, Foster City, CA); GeneRuler 1-kb DNA ladder (MBI Fermentas, Hanover, MD); rat genomic DNA (ATCC, Manassas, VA); and qPCR primers (SuperArray, Frederick, MD). ..

    Article Title: RNA Editing of Androgen Receptor Gene Transcripts in Prostate Cancer Cells
    Article Snippet: .. The following reagents were purchased: AR agonist R1881 (methyltrienolone) (PerkinElmer Life Sciences); 17 -estradiol, progesterone, cyproterone acetate (CPA), hydroxyflutamide (HF) (Sigma); double-stranded siRNAs (Dharmacon Research, Lafayette, CO);Oligofectamine reagent, 4–12%SDS-polyacrylamide gels, and the TOPO TA cloning kit (Invitrogen); prestained Precision Plus protein standards and goat antimouse horseradish peroxidase-conjugated secondary (BioRad); mouse monoclonal AR antibody (AR441) (Santa Cruz Biotechnology (Santa Cruz, CA);MEGAscript high yield transcription kit (Ambion, Austin, TX); BCA protein assay kit (Pierce); ECL reagents kit and Hyperfilm ECL film (GE Healthcare); RNeasy midi kit, Oligotex Midi kit, and DNA oligonucleotides (Qiagen, Valencia, CA); proteinase K solution, DNase-free RNase, transcriptor reverse transcriptase enzyme, and FastStart TaqDNA polymerase (Roche Applied Science); fetal bovine serum and charcoal-stripped fetal bovine serum (Hyclone Laboratories, Logan, UT); fluorescent short tandem repeat (STR) oligonucleotides (Applied Biosystems, Foster City, CA); GeneRuler 1-kb DNA ladder (MBI Fermentas, Hanover, MD); rat genomic DNA (ATCC, Manassas, VA); and qPCR primers (SuperArray, Frederick, MD). ..

    Article Title: RNA Editing of Androgen Receptor Gene Transcripts in Prostate Cancer Cells
    Article Snippet: .. Reagents The following reagents were purchased: AR agonist R1881 (methyltrienolone) (PerkinElmer Life Sciences); 17β-estradiol, progesterone, cyproterone acetate (CPA), hydroxyflutamide (HF) (Sigma); double-stranded siRNAs (Dharmacon Research, Lafayette, CO); Oligofectamine reagent, 4–12% SDS-polyacrylamide gels, and the TOPO TA cloning kit (Invitrogen); prestained Precision Plus protein standards and goat anti-mouse horseradish peroxidase-conjugated secondary (Bio-Rad); mouse monoclonal AR antibody (AR441) (Santa Cruz Biotechnology (Santa Cruz, CA); MEGAscript® high yield transcription kit (Ambion, Austin, TX); BCA protein assay kit (Pierce); ECL reagents kit and Hyperfilm ECL film (GE Healthcare); RNeasy midi kit, Oligotex Midi kit, and DNA oligonucleotides (Qiagen, Valencia, CA); proteinase K solution, DNase-free RNase, transcriptor reverse transcriptase enzyme, and FastStart Taq DNA polymerase (Roche Applied Science); fetal bovine serum and charcoal-stripped fetal bovine serum (Hyclone Laboratories, Logan, UT); fluorescent short tandem repeat (STR) oligonucleotides (Applied Biosystems, Foster City, CA); GeneRuler 1-kb DNA ladder (MBI Fermentas, Hanover, MD); rat genomic DNA (ATCC, Manassas, VA); and qPCR primers (SuperArray, Frederick, MD). ..

    Real-time Polymerase Chain Reaction:

    Article Title: RNA Editing of Androgen Receptor Gene Transcripts in Prostate Cancer Cells
    Article Snippet: .. The following reagents were purchased: AR agonist R1881 (methyltrienolone) (PerkinElmer Life Sciences); 17β-estradiol, progesterone, cyproterone acetate (CPA), hydroxyflutamide (HF) (Sigma); double-stranded siRNAs (Dharmacon Research, Lafayette, CO); Oligofectamine reagent, 4–12% SDS-polyacrylamide gels, and the TOPO TA cloning kit (Invitrogen); prestained Precision Plus protein standards and goat anti-mouse horseradish peroxidase-conjugated secondary (Bio-Rad); mouse monoclonal AR antibody (AR441) (Santa Cruz Biotechnology (Santa Cruz, CA); MEGAscript® high yield transcription kit (Ambion, Austin, TX); BCA protein assay kit (Pierce); ECL reagents kit and Hyperfilm ECL film (GE Healthcare); RNeasy midi kit, Oligotex Midi kit, and DNA oligonucleotides (Qiagen, Valencia, CA); proteinase K solution, DNase-free RNase, transcriptor reverse transcriptase enzyme, and FastStart Taq DNA polymerase (Roche Applied Science); fetal bovine serum and charcoal-stripped fetal bovine serum (Hyclone Laboratories, Logan, UT); fluorescent short tandem repeat (STR) oligonucleotides (Applied Biosystems, Foster City, CA); GeneRuler 1-kb DNA ladder (MBI Fermentas, Hanover, MD); rat genomic DNA (ATCC, Manassas, VA); and qPCR primers (SuperArray, Frederick, MD). ..

    Article Title: RNA Editing of Androgen Receptor Gene Transcripts in Prostate Cancer Cells
    Article Snippet: .. The following reagents were purchased: AR agonist R1881 (methyltrienolone) (PerkinElmer Life Sciences); 17 -estradiol, progesterone, cyproterone acetate (CPA), hydroxyflutamide (HF) (Sigma); double-stranded siRNAs (Dharmacon Research, Lafayette, CO);Oligofectamine reagent, 4–12%SDS-polyacrylamide gels, and the TOPO TA cloning kit (Invitrogen); prestained Precision Plus protein standards and goat antimouse horseradish peroxidase-conjugated secondary (BioRad); mouse monoclonal AR antibody (AR441) (Santa Cruz Biotechnology (Santa Cruz, CA);MEGAscript high yield transcription kit (Ambion, Austin, TX); BCA protein assay kit (Pierce); ECL reagents kit and Hyperfilm ECL film (GE Healthcare); RNeasy midi kit, Oligotex Midi kit, and DNA oligonucleotides (Qiagen, Valencia, CA); proteinase K solution, DNase-free RNase, transcriptor reverse transcriptase enzyme, and FastStart TaqDNA polymerase (Roche Applied Science); fetal bovine serum and charcoal-stripped fetal bovine serum (Hyclone Laboratories, Logan, UT); fluorescent short tandem repeat (STR) oligonucleotides (Applied Biosystems, Foster City, CA); GeneRuler 1-kb DNA ladder (MBI Fermentas, Hanover, MD); rat genomic DNA (ATCC, Manassas, VA); and qPCR primers (SuperArray, Frederick, MD). ..

    Article Title: RNA Editing of Androgen Receptor Gene Transcripts in Prostate Cancer Cells
    Article Snippet: .. Reagents The following reagents were purchased: AR agonist R1881 (methyltrienolone) (PerkinElmer Life Sciences); 17β-estradiol, progesterone, cyproterone acetate (CPA), hydroxyflutamide (HF) (Sigma); double-stranded siRNAs (Dharmacon Research, Lafayette, CO); Oligofectamine reagent, 4–12% SDS-polyacrylamide gels, and the TOPO TA cloning kit (Invitrogen); prestained Precision Plus protein standards and goat anti-mouse horseradish peroxidase-conjugated secondary (Bio-Rad); mouse monoclonal AR antibody (AR441) (Santa Cruz Biotechnology (Santa Cruz, CA); MEGAscript® high yield transcription kit (Ambion, Austin, TX); BCA protein assay kit (Pierce); ECL reagents kit and Hyperfilm ECL film (GE Healthcare); RNeasy midi kit, Oligotex Midi kit, and DNA oligonucleotides (Qiagen, Valencia, CA); proteinase K solution, DNase-free RNase, transcriptor reverse transcriptase enzyme, and FastStart Taq DNA polymerase (Roche Applied Science); fetal bovine serum and charcoal-stripped fetal bovine serum (Hyclone Laboratories, Logan, UT); fluorescent short tandem repeat (STR) oligonucleotides (Applied Biosystems, Foster City, CA); GeneRuler 1-kb DNA ladder (MBI Fermentas, Hanover, MD); rat genomic DNA (ATCC, Manassas, VA); and qPCR primers (SuperArray, Frederick, MD). ..



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